flowio

Topics in the source

• FlowIO: Flow Cytometry Standard File Handler
• Overview
• When to Use This Skill
• Installation
• Quick Start
• Basic File Reading
• Creating FCS Files
• Core Workflows

FlowIO is a lightweight Python library for reading and writing Flow Cytometry Standard (FCS) files. Parse FCS metadata, extract event data, and create new FCS files with minimal dependencies. The library supports FCS versions 2.0, 3.0, and 3.1, making it ideal for backend services, data pipelines, and basic cytometry file operations. This skill should be used when: FCS files requiring parsing or metadata extraction Flow cytometry data needing conversion to NumPy arrays Event data requiring export to FCS format Multi-dataset FCS files needing separation Channel information extraction (scatter, fluorescence, time) Cytometry file validation or inspection Pre-processing workflows before advanced analysis Related Tools: For advanced flow cytometry analysis including compensation, gating, and FlowJo/GatingML support, recommend FlowKit library as a companion to FlowIO. Requires Python 3.9 or later. The FlowData class provides the primary interface for reading FCS files. Standard Reading: Memory-Efficient Metadata Reading: When only metadata is needed (no event data): Handling Problematic Files: Some FCS files have offset discrepancies or errors: Excluding Null Channels: FCS files contain

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